A STUDY ON PHARMACOLOGICAL ACTIVITIES, NUTRITIONAL VALUE AND DNA BARCODING OF SELECTED ETHNOMEDICINAL PLANTS FROM GOALPARA DISTRICT, ASSAM

Abstract

studies. Methanolic and hexane solvents were used for extraction of secondary compounds. Preliminary phytochemical analysis revealed the presence of alkaloid, flavonoid, phenolic compounds, tannin and triterpenoids whereas saponin was absent in both of the extracts. Presence of the above mentioned compounds have very potent antimicrobial property. Highest phenolic content was found in methanolic extract of D. picta (112.33±0.13 mg GAE g -1 DW) followed by D. papillulifera (73.84±3.19 mg GAE g -1 DW) and highest flavonoid content was found in methanolic extract of D. papillulifera (26.54±0.67 mg QE g -1 DW) followed by D. picta (21.23±1.35 mg QE g -1 DW). For antimicrobial activity, Pyricularia oryzae (MTCC 1477), Xanthomonas oryzae (MTCC 11102), Colletotrichum gloesporoides (MTCC 10529) and Sclerotonia slerotium (MTCC 8785). were considered. This study revealed that methanolic extract of D. picta showed potential antimicrobial activity against phytopathogens of Rice and Jute crop plants viz. Pyricularia oryzae and Colletotrichum gloesporoides. The extracts were effective in inhibiting the mycelial growth of phytopathogenic fungi and the vulnerability to the extract was in the order: P. oryzae > C. gloesporoides > X. oryzae > S. slerotium. The Minimal inhibitory concentrations and IC50 values of the tested foliose lichens against tested phytopathogens. The MIC values of the methanolic extracts were lower than that of hexane extracts. In future further research on secondary metabolites of D. picta could lead to use the lichen as a potent biological control measurement as fungiside. Keywords: Lichen Biodiversity, North-East India, Endemic, New records, Phytochemical screening, Phytopathogens, Antimicrobial screening.studies. Methanolic and hexane solvents were used for extraction of secondary compounds. Preliminary phytochemical analysis revealed the presence of alkaloid, flavonoid, phenolic compounds, tannin and triterpenoids whereas saponin was absent in both of the extracts. Presence of the above mentioned compounds have very potent antimicrobial property. Highest phenolic content was found in methanolic extract of D. picta (112.33±0.13 mg GAE g -1 DW) followed by D. papillulifera (73.84±3.19 mg GAE g -1 DW) and highest flavonoid content was found in methanolic extract of D. papillulifera (26.54±0.67 mg QE g -1 DW) followed by D. picta (21.23±1.35 mg QE g -1 DW). For antimicrobial activity, Pyricularia oryzae (MTCC 1477), Xanthomonas oryzae (MTCC 11102), Colletotrichum gloesporoides (MTCC 10529) and Sclerotonia slerotium (MTCC 8785). were considered. This study revealed that methanolic extract of D. picta showed potential antimicrobial activity against phytopathogens of Rice and Jute crop plants viz. Pyricularia oryzae and Colletotrichum gloesporoides. The extracts were effective in inhibiting the mycelial growth of phytopathogenic fungi and the vulnerability to the extract was in the order: P. oryzae > C. gloesporoides > X. oryzae > S. slerotium. The Minimal inhibitory concentrations and IC50 values of the tested foliose lichens against tested phytopathogens. The MIC values of the methanolic extracts were lower than that of hexane extracts. In future further research on secondary metabolites of D. picta could lead to use the lichen as a potent biological control measurement as fungiside. Keywords: Lichen Biodiversity, North-East India, Endemic, New records, Phytochemical screening, Phytopathogens, Antimicrobial screening.studies. Methanolic and hexane solvents were used for extraction of secondary compounds. Preliminary phytochemical analysis revealed the presence of alkaloid, flavonoid, phenolic compounds, tannin and triterpenoids whereas saponin was absent in both of the extracts. Presence of the above mentioned compounds have very potent antimicrobial property. Highest phenolic content was found in methanolic extract of D. picta (112.33±0.13 mg GAE g -1 DW) followed by D. papillulifera (73.84±3.19 mg GAE g -1 DW) and highest flavonoid content was found in methanolic extract of D. papillulifera (26.54±0.67 mg QE g -1 DW) follostudies. Methanolic and hexane solvents were used for extraction of secondary compounds. Preliminary phytochemical analysis revealed the presence of alkaloid, flavonoid, phenolic compounds, tannin and triterpenoids whereas saponin was absent in both of the extracts. Presence of the above mentioned compounds have very potent antimicrobial property. Highest phenolic content was found in methanolic extract of D. picta (112.33±0.13 mg GAE g -1 DW) followed by D. papillulifera (73.84±3.19 mg GAE g -1 DW) and highest flavonoid content was found in methanolic extract of D. papillulifera (26.54±0.67 mg QE g -1 DW) followed by D. picta (21.23±1.35 mg QE g -1 DW). For antimicrobial activity, Pyricularia oryzae (MTCC 1477), Xanthomonas oryzae (MTCC 11102), Colletotrichum gloesporoides (MTCC 10529) and Sclerotonia slerotium (MTCC 8785). were considered. This study revealed that methanolic extract of D. picta showed potential antimicrobial activity against phytopathogens of Rice and Jute crop plants viz. Pyricularia oryzae and Colletotrichum gloesporoides. The extracts were effective in inhibiting the mycelial growth of phytopathogenic fungi and the vulnerability to the extract was in the order: P. oryzae > C. gloesporoides > X. oryzae > S. slerotium. The Minimal inhibitory concentrations and IC50 values of the tested foliose lichens against tested phytopathogens. The MIC values of the methanolic extracts were lower than that of hexane extracts. In future further research on secondary metabolites of D. picta could lead to use the lichen as a potent biological control measurement as fungiside. Keywords: Lichen Biodiversity, North-East India, Endemic, New records, Phytochemical screening, Phytopathogens, Antimicrobial screening.studies. Methanolic and hexane solvents were used for extraction of secondary compounds. Preliminary phytochemical analysis revealed the presence of alkaloid, flavonoid, phenolic compounds, tannin and triterpenoids whereas saponin was absent in both of the extracts. Presence of the above mentioned compounds have very potent antimicrobial property. Highest phenolic content was found in methanolic extract of D. picta (112.33±0.13 mg GAE g -1 DW) followed by D. papillulifera (73.84±3.19 mg GAE g -1 DW) and highest flavonoid content was found in methanolic extract of D. papillulifera (26.54±0.67 mg QE g -1 DW) followed by D. picta (21.23±1.35 mg QE g -1 DW). For antimicrobial activity, Pyricularia oryzae (MTCC 1477), Xanthomonas oryzae (MTCC 11102), Colletotrichum gloesporoides (MTCC 10529) and Sclerotonia slerotium (MTCC 8785). were considered. This study revealed that methanolic extract of D. picta showed potential antimicrobial activity against phytopathogens of Rice and Jute crop plants viz. Pyricularia oryzae and Colletotrichum gloesporoides. The extracts were effective in inhibiting the mycelial growth of phytopathogenic fungi and the vulnerability to the extract was in the order: P. oryzae > C. gloesporoides > X. oryzae > S. slerotium. The Minimal inhibitory concentrations and IC50 values of the tested foliose lichens against tested phytopathogens. The MIC values of the methanolic extracts were lower than that of hexane extracts. In future further research on secondary metabolites of D. picta could lead to use the lichen as a potent biological control measurement as fungiside. Keywords: Lichen Biodiversity, North-East India, Endemic, New records, Phytochemical screening, Phytopathogens, Antimicrobial screening.wed by D. picta (21.23±1.35 mg QE g -1 DW). For antimicrobial activity, Pyricularia oryzae (MTCC 1477), Xanthomonas oryzae (MTCC 11102), Colletotrichum gloesporoides (MTCC 10529) and Sclerotonia slerotium (MTCC 8785). were considered. This study revealed that methanolic extract of D. picta showed potential antimicrobial activity against phytopathogens of Rice and Jute crop plants viz. Pyricularia oryzae and Colletotrichum gloesporoides. The extracts were effective in inhibiting the mycelial growth of phytopathogenic fungi and the vulnerability to the extract was in the order: P. oryzae > C. gloesporoides > X. oryzae > S. slerotium. The Minimal inhibitory concentrations and IC50 values of the tested foliose lichens against tested phytopathogens. The MIC values of the methanolic extracts were lower than that of hexane extracts. In future further research on secondary metabolites of D. picta could lead to use the lichen as a potent biological control measurement as fungiside. Keywords: Lichen Biodiversity, North-East India, Endemic, New records, Phytochemical screening, Phytopathogens, Antimicrobial screening.